Cyclic AMP ELISA Assay Kit: A Sensitive and Comprehensive Tool for Quantifying Cyclic Adenosine Monophosphate

Cyclic adenosine monophosphate (cAMP) is a crucial 'second messenger' that plays a significant role in various physiological processes, including the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism. To accurately quantify cAMP levels, researchers often rely on sensitive and reliable assay kits. Among these, the Cyclic AMP ELISA Assay Kit (ab65355) stands out for its exceptional sensitivity and comprehensive design.

This colorimetric competitive ELISA kit is capable of detecting cAMP levels as low as 0.006 pmol/ml in intracellular samples and 0.027 pmol/ml in extracellular samples. The kit's high sensitivity makes it an ideal choice for researchers working with a wide range of sample types, including cell lysate, tissue, and urine.

The Cyclic AMP ELISA Assay Kit uses a competitive binding mechanism to quantify cAMP levels. This approach involves incubating the sample with a fixed amount of enzyme-conjugated antibody, which competes with endogenous cAMP for binding sites on the solid phase. The resulting colorimetric signal is directly proportional to the concentration of cAMP in the sample.

This kit is designed to be highly specific, with minimal cross-reactivity with other nucleotides such as ATP, cyclic GMP, GMP, GTP, cyclic UMP, and CTP (less than 0.001%). The kit's components are carefully optimized to ensure accurate and reliable results, including the GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 2, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, and Triethylamine.

The Cyclic AMP ELISA Assay Kit is compatible with the Absorbance 96 Plate Reader and can be used for quantitative determination of cAMP in culture supernatants, serum, and saliva from any species. The kit's comprehensive design makes it an essential tool for researchers investigating various biological processes involving cAMP signaling.

In summary, the Cyclic AMP ELISA Assay Kit (ab65355) is a highly sensitive and reliable tool for quantifying cyclic adenosine monophosphate levels in samples from mammals and other species. Its exceptional sensitivity, specificity, and comprehensive design make it an ideal choice for researchers working with cAMP-related biological processes.

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